A  B  C  D  E  F  G  H  I  J  K  L  M  N  O  P  Q  R  S  T  U  V  W  X  Y  Z  

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Acceptable Specimen: Pure isolate in appropriate tubed transport medium or Amies (with charcoal) transport medium. Plates not accepted.

Stability: Ambient: 7 days; Refrigerated: 7 days; Frozen: Unacceptable

Normal Value: Not applicable

Turn Around Time: 7 days

Acceptable Specimen: Pure isolate in appropriate tubed transport medium or Amies (with charcoal) transport medium. Plates not accepted.

Stability: Ambient: 7 days; Refrigerated: 7 days; Frozen: Unacceptable

Normal Value: Not applicable

Turn Around Time: 7 days

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 5 Days; Refrigerated: 5 Days; Frozen: >5 Days

Normal Value: West Nile Virus EIA - Negative; Western Equine, Eastern Equine, St. Louis, California Group IFA < 1:16

Turn Around Time: 3 days

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Acceptable Specimen: Isolate in appropriate tubed transport medium or cut out a piece of agar with growth and send in a sterile container. Plates are not accepted. Cutaneous collect in vesicular fluid with sterile swab or collect from beneath the eschar. Gastrointestinal collect blood, stool, or rectal swabs. Inhalational collect blood.

Transport: Cutaneous, gastrointestinal and inhalational at room temperature.

Turn Around Time: 1 to 2 days

Acceptable Specimen: Nasopharyngeal specimen on provided kit swab.

Click here for collection instructions.

Click here for collection kits.

Stability: Ambient: 7 days; Refrigerated: 7 days; Frozen: Unacceptable

Normal Value: Negative

Turn Around Time: 3 days

Acceptable Specimen: 2 ml acute and convalescent sample (3 weeks post onset) phase sera recommended

Stability: Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: >4 days

Normal Value: Negative

Turn Around Time: 2 days

Acceptable Specimen: Isolate in appropriate tubed transport medium or cut out a piece of agar with growth and send in a sterile container. Plates are not accepted. For primary isolation and PCR, collect blood or bone marrow

Transport: Room temperature

Turn Around Time: 3 to 5 days

Acceptable Specimen: Isolate in appropriate tubed transport medium or cut out a piece of agar with growth and send in a sterile container. Plates are not accepted.

Transport: Blood, bone marrow, sputum, abscess and wound swabs, urine transport blood room temp; all others transport at 2 to 8°C

Turn Around Time: 1 to 2 days

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Refer to Arboviral Encephalitis Panel

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

*Facilities using molecular methods without conventional culture may send original stool sample.

Since isolate submission is no longer required, facilities requesting confirmation of Campylobacter species will be charged for an Aerobic ID.

A report indicating Campylobacter jejuni or Campylobacter species will be issued to the submitting facility.

Acceptable Specimen: Rectal swab specimen collected with BD BBL liquid Stuart’s dual culture swab

Stability: Ambient: 5 days; Refrigerated: 5 days; Frozen: Unacceptable

Normal Value: KPC, NDM, VIM, OXA-48, and IMP Not Detected

Turn Around Time: 1 day

Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

*Please submit antimicrobial susceptibility results from instrument printout.

A report containing test results to support infection prevention measures will be issued to the submitting facility.

Acceptable Specimen: Obtain epithelial cells from infected site. Place swab specimen into viral transport medium immediately.

Stability: Refrigerated: 2 days; Frozen: >2 days

Normal Value: Negative

Turn Around Time: 7 days

Acceptable Specimen: Endocervical, oral, rectal, urine, vaginal, or male urethral specimen in Abbott multi specimen collection kit

  • Patient must not urinate for at least 1 hour prior to sampling
  • Collect 20 to 30 mls of a first-catch urine
  • Use the transfer pipette provided to fill the collection tube to a volume within the two black lines on the side of the tube

Click here for collection instructions.

Click here for collection kits.

Stability: Ambient: 14 days; Refrigerated: 14 days; Frozen: >14 days

Normal Value: Negative

Turn Around Time: 1 day

Acceptable Specimen: Contact the Division of Microbiology for specific recommendation regarding collection and transportation.

All testing will be performed by the Minnesota Department of Health and/or the Centers for Disease Control and Prevention.

Turn Around Time: Procedure dependent

Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

A report with the organism identification will be issued to the submitting facility.

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: <1:8

Turn Around Time: 1 day

Acceptable Specimen: Throat swab and biopsy in viral transport medium. CSF, urine, bronchial specimens, amniotic fluid and plasma in sterile container.

Stability: Refrigerated: 7 days; Frozen: >7 days

Normal Value: Negative

Turn Around Time: 3 days

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Refer to Arboviral Encephalitis Panel

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: <1:8 or Negative

Turn Around Time: 1 day

Acceptable Specimen: Nasopharyngeal, skin lesion material, and throat in viral transport medium. CSF and stool in sterile container.

Stability: Refrigerated: 7 days; Frozen: >7 days

Normal Value: Negative

Turn Around Time: 3 days

Specimen for PCR testing will be referred to a reference laboratory.

Acceptable Specimen: Nasopharyngeal, oropharyngeal swab, nasal wash, nasal aspirate in viral transport medium.

Stability: Refrigerated: 7 days; Frozen: >7 days

Normal Value: Negative

Turn Around Time: 3 days

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: <1:8

Turn Around Time: 1 day

Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

*Facilities using molecular methods without conventional culture may send original stool sample.

A report with serotyping results will be issued to the submitting facility.

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Acceptable Specimen: 2 ml serum

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: Nonreactive

Turn Around Time: 2 days

Acceptable Specimen: 2 ml acute and convalescent (3 weeks post onset) phase sera

Stability: Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: >4 hours

Normal Value: Negative

Turn Around Time: 2 days

Acceptable Specimen: Isolate in appropriate tubed transport medium or cut out a piece of agar with growth and send in a sterile container. Plates are not accepted. For primary isolation and PCR collect blood, biopsied tissue or ulcer scraping.

Transport: Blood at room temperature. Tissue and ulcer samples at 2 to 8°C

Turn Around Time: 2 to 4 days

Acceptable Specimen: Sterile containers or on Sabouraud Dextrose Agar. Plates will not be accepted. Contact the Division of Microbiology for assistance with systemic isolates such as histoplasma, coccidioides and blastomyces.

Stability: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable

Normal Value: No fungi isolated

Turn Around Time: Negative culture - 2 weeks; Positive culture - 2 to 6 weeks

Acceptable Specimen: Pure isolate in tubed medium such as Sabouraud Dextrose Agar or Amies transport medium. Plates not accepted. Contact the Division of Microbiology for assistance with systemic isolates such as histoplasma, coccidioides and blastomyces.

Stability: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable

Normal Value: Not applicable

Turn Around Time: 2 to 6 weeks

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Acceptable Specimen: 1 ml raw stool or 1 ml stool in Cary-Blair medium

This assay simultaneously tests for the following gastric pathogens: Adenovirus (40/41), Norovirus (GI/GII), Rotavirus A, Campylobacter, Clostridium difficile toxin (A/B), Escherichia coli O157, Enterotoxigenic E. coli (ETEC)(LT/ST), Salmonella, Shiga-like Toxin producing E. coli (stx1/stx2), Shigella, Vibrio cholerae toxin gene (ctx), Cryptosporidium, Entamoeba histolytica, and Giardia lamblia

Stability: Raw Stool - Ambient: Unacceptable; Refrigerated: 2 days; Frozen: 2 days

Stability: Stool in Cary Blair - Ambient: 2 days; Refrigerated: 2 days; Frozen: Unacceptable

Normal Value: Not Detected

Turn Around Time: 2 days

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Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

The isolate may be forwarded to Minnesota Public Health Laboratory for testing.

A report with serotyping results will be issued to the submitting facility.

Handling fees are dependent on test requested. Call the Division of Microbiology for test specific handling fee information.

Acceptable Specimen: 2 ml acute phase serum

Stability: Refrigerated: 5 days; Frozen: >5 days

Normal Value: Negative

*Positive results are sent to CDC for Confirmation.

Turn Around Time: 2 days

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

Turn Around Time: 1 day

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 1 day; Refrigerated: 6 days; Frozen: >6 days

Normal Value: Nonreactive

Turn Around Time: 1 day

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 1 day; Refrigerated: 6 days; Frozen: >6 days

Normal Value: Nonreactive

Turn Around Time: 1 day

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Reactive

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum

Stability: Ambient: 1 day; Refrigerated: 6 days; Frozen: >6 days

Normal Value: Nonreactive

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

*Positive tests will reflex to Hepatitis C Virus RNA

Turn Around Time: 1 day

Acceptable Specimen: 3 ml serum or 5 ml whole blood

Stability: Serum - Ambient: 1 day; Refrigerated: 3 days; Frozen: >3 days

Stability: Whole Blood - Ambient: 6 hours; Refrigerated: 6 hours; Frozen: Unacceptable

Normal Value: Not Applicable

Turn Around Time: Wednesday

Acceptable Specimen: 3 ml serum or 5 ml whole blood

Stability: Serum - Ambient: 1 day; Refrigerated: 3 days; Frozen: >3 days

Stability: Whole Blood - Ambient: 6 hours; Refrigerated: 6 hours; Frozen: Unacceptable

Normal Value: Not Applicable

Turn Around Time: Tuesday

Acceptable Specimen: Genital, urethral, oral, tissue, and vesicle in viral transport medium. CSF in sterile container.

Stability: Refrigerated: 10 days; Frozen: >10 days

Normal Value: Negative

Turn Around Time: Tuesday and Friday

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: Negative

Turn Around Time: 1 day

Acceptable Specimen: 3 ml serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

*Confirmatory testing will be performed, at no additional charge, on all reactive specimens following current CDC and CLSI guidelines for 4th Generation HIV Ab/Ag Combo tests.

Turn Around Time: 1 day

Acceptable Specimen: 1 ml serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

Turn Around Time: 2 days

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Acceptable Specimen: 2 ml serum

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Measles IgG > 1:16
  Mumps IgG > 1:16
  Rubella - Immune

Turn Around Time: Tuesday and Thursday

Acceptable Specimen: Specimen in viral transport medium. Refer to Specimen Collection and Handling Section for instructions.

Click here for collection instructions.

Click here for collection kits.

Sub-typing: A - H3, 2009H1N1, H5, H7

Sub-typing: B - Yamagata, Victoria

Stability: Refrigerated: 7 days; Frozen: >7 days

Normal Value: Not Applicable

Turn Around Time: 3 days

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Acceptable Specimen: Bronchial, lung, sputum, tissue in sterile container

Stability: Ambient: Unacceptable; Refrigerated: 7 days; Frozen: Unacceptable

Normal Value: Negative

Turn Around Time: 7 days

Acceptable Specimen: 2 ml of serum

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: Negative

*Positive & Equivocal results are sent to CDC for Confirmation.

Turn Around Time: 2 days

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Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Whole blood sample in EDTA prior to treatment plus thick and thin blood films made from fresh blood or EDTA (if prepared within one hour) if available.

The sample will be forwarded to CDC for species confirmation and evaluation of emerging drug resistance.

A report will be issued to the submitting facility.

Acceptable Specimen: 2 ml acute phase serum. Include immunization history on test request form.

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: <1:8

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum. Include immunization history on test request form.

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: >1:16

Turn Around Time: Tuesday and Thursday

Acceptable Specimen: Nasopharyngeal swabs or aspirates, nasal swabs, throat swabs, buccal swabs in viral transport medium. Urine in sterile container.

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Negative

Turn Around Time: 2 days

Acceptable Specimen: 2 ml acute phase serum. Include immunization history on test request form.

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: <1:8

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum. Include immunization history on test request form.

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: >1:16

Turn Around Time: Tuesday and Thursday

Acceptable Specimen: Buccal swabs (preferred), throat swabs, and nasopharyngeal swabs or aspirates in viral transport medium. Urine in sterile container.

Click here for collection instructions

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Negative

Turn Around Time: 2 days

Acceptable Specimen: Sterile container

Contact the Division of Microbiology for collection and mailing kit. Click here for Specimen Collection and Handling instructions.

Stability: Whole Blood/Bone Marrow - Ambient: 7 days; Frozen: Unacceptable

Stability: Other - Ambient: Unacceptable; Refrigerated: 7 days; Frozen: Unacceptable

Normal Value: Mycobacteria Culture - No Mycobacteria isolated; AFB Smear - No AFB seen

Turn Around Time: 2 to 8 weeks

Acceptable Specimen: Isolate on tubed solid medium such as LJ slants, 7H10 slants, etc. Plates or liquid medium not accepted.

Stability: Ambient: 4 days; Refrigerated: 7 days fresh growth; Frozen: Unacceptable

Normal Value: Not Applicable

Turn Around Time: 2 to 6 weeks

Isolates for susceptibility testing will be referred to a reference laboratory.

Susceptibility testing on M .tuberculosis will be submitted on all isolates from patients considered to be a new case as part of the initial culture procedure.

Susceptibility testing on mycobacteria isolates other than M. tuberculosis will be submitted to a reference laboratory upon request.

Normal Value: Susceptible

Turn Around Time: Not Applicable

Acceptable Specimen: 1 ml Sputum

2 samples per patient in a 3 month period or 12 months after the end of treatment.

The PCR test is only intended for use on specimens from patients showing signs and symptoms consistent with active pulmonary tuberculosis. The PCR test must be performed in conjunction with mycobacterial culture.

Stability: Ambient: 3 days; Refrigerated: 10 days; Frozen: Unacceptable

Normal Value: MTB Not detected

Turn Around Time: Test performed as needed

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: <1:64

Turn Around Time: 1 day

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Can only be ordered as part of a dual test for Chlamydia trachomatis and Neisseria gonorrhoeae.

Refer to Chlamydia trachomatis and Neisseria gonorrhoeae RT-PCR

Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

The isolate may be forwarded to Minnesota Public Health Laboratory for testing.

A report with serotyping results will be issued to the submitting facility.

Acceptable Specimen: 1-10 ml feces or vomitus 2 to 3 days after symptom onset

Stability: Refrigerated: 3 days

Normal Value: Negative

Turn Around Time: 3 days

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Acceptable Specimen: At least two thin blood films and two thick films made from fresh blood are preferred

Blood containing anticoagulant (EDTA) can be used if films are prepared within one hour.

Air dry and send in protected container to prevent breakage.

The submission of a single blood specimen will not rule out blood parasites. Additional blood specimens are recommended.

Include EDTA whole blood and serum tubes for possible referral.

Pertinent travel history is requested.

Stability: Ambient: 7 days; Frozen: Unacceptable

Normal Value: No parasites seen

Turn Around Time: 2 days

Acceptable Specimen: Stool specimen in Total-Fix preservative includes wet mount, trichrome stain and acid-fast stain (for Cryptosporidium, Cyclospora and Cystoisospora belli). Microsporidia examination is not included. If fresh stool sample is collected, sample must be placed in Total-Fix collection container within 30 minutes.

Two specimens are recommended, while three specimens offer the best chance of organism recovery.

Antibiotics such as metronidazole or tetracycline may interfere with the recovery of intestinal parasites, particularly the protozoa.

If specimen is collected in formalin, trichrome stain will not be performed.

*If Schistosomiasis is suspected, please send urine and stool specimens.

Click here for collection kits.

Stability: Ambient: 7 days; Frozen: Unacceptable

Normal Value: No parasites seen

Turn Around Time: 2 days

Refer to Bordetella pertussis PCR

Acceptable Specimen: 2 ml serum

Stability: Ambient: 1 day; Refrigerated: 6 days; Frozen: >6 days

Normal Value: Nonreactive

Turn Around Time: 1 day

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Serum for IFA testing will be referred to a reference laboratory.

Acceptable Specimen: 2 ml acute and convalescent (3 weeks post onset) phase sera

Stability: Ambient: 1 day; Refrigerated: 6 days; Frozen: >6 days

Normal Value: < 1:256 or a less than fourfold increase in titer between acute and convalescent sera.

Turn Around Time: 7 days

Acceptable Specimen: Three tube blood collection kit

Click here for collection instructions

Click here for collection kits.

Samples must be received Monday through Thursday.

Stability: Refer to Collection Instructions link above

Normal Value: Negative

Turn Around Time: 3 days

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Acceptable Specimen: Appropriate brain tissue. Contact the Division of Microbiology for assistance.

Please submit a rabies request form for each specimen.

Please call for consultation if animal brain cannot be removed prior to submission.

*50.00 charge for brain extraction for animals larger than a rodent (ex. Bat, squirrel, etc)

Stability: Ambient: Unacceptable; Refrigerated: 2 days; Frozen: Unacceptable

Normal Value: Negative

Turn Around Time: 1 day

Acceptable Specimen: Nasopharyngeal swabs in viral transport medium

This assay simultaneously tests for the following respiratory pathogens: Influenza A, Influenza B, Respiratory Syncytial Virus (RSV) A & B, Parainfluenza 1, 2,3 &4, Human Bocavirus, Human Metapneumovirus, Human Rhinovirus/Enterovirus, Adenovirus, Coronavirus HKU1, NL63, OC43, 229E, Chlamydophila pneumoniae and Mycoplasma pneumoniae.

Stability: Refrigerated: 7 days; Frozen: >7 days

Normal Value: Negative

Turn Around Time: 3 days

Acceptable Specimen: 2 ml serum

Stability: Ambient: Unacceptable; Refrigerated: 5 days; Frozen: >5 days

Normal Value: Nonreactive

*Reactive RPR will be confirmed by TP-PA at no additional charge.

Turn Around Time: Nonreactive - 1 day; Reactive - Tuesday and Thursday

Acceptable Specimen: Liquid, soil, powder, wipes, swabs, paper, plant material and food samples

Turn Around Time: 1 day

Acceptable Specimen: Venous whole blood preserved with EDTA or acid citrate dextrose Solution A (ACD-A)

This assay is intended for the qualitative detection and differentiation of R. rickettsii and R. prowazekii DNA. Specimens can be collected from individuals with signs or symptoms indicative of Rocky Mountain Spotted Fever or epidemic typhus and epidemiological risk factors consistent with potential exposure.

Stability: Ambient: Unacceptable; Refrigerated: 7 days; Frozen: Unacceptable

Normal Value: Negative

Turn Around Time: 2 days

Acceptable Specimen: 2 ml acute and convalescent (3 weeks post onset) phase sera

Stability: Ambient: 1 day; Refrigerated: 3 days; Frozen: >3 days

Normal Value: < 1:64 or less than fourfold increase in titer between acute and convalescent sera.

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

*Reactive RPR can be confirmed by TP-PA at facilities request. Contact the Division of Microbiology to order.

Turn Around Time: Nonreactive - 1 day; Reactive - Tuesday and Thursday

Acceptable Specimen: 2 ml acute phase serum. Include immunization history on test request form.

Stability: Refrigerated: 2 days; Frozen: >2 days

Normal Value: Negative

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum

Stability: Refrigerated: 8 days; Frozen: >8 days

Normal Value: Immune

Turn Around Time: Tuesday and Thursday

Refer to Measles Virus

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Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

*Facilities using molecular methods without conventional culture may send original stool sample.

A report with serotyping results will be issued to the submitting facility.

Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Shiga toxin positive specimen in appropriate transport media (MacConkey broth, GN broth, etc.)

*Facilities using molecular methods without conventional culture may send original stool sample.

A report indicating if Shiga toxin-producing Escherichia coli was isolated will be issued to the submitting facility.

Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

*Facilities using molecular methods without conventional culture may send original stool sample.

A report with serotyping results will be issued to the submitting facility.

Acceptable Specimen: Contact the Division of Microbiology for specific recommendation regarding collection and transportation.

Specimens to collect include: Fluid and cells from two or more unroofed vesicles/pustules; a minimum of four touch preparation slides; two to four synthetic swabs in viral transport medium.

Call the North Dakota Department of Health if you suspect smallpox, an adverse reaction to smallpox vaccination or require consultation on an unusual or pustular rash illness.

Confirmation for Smallpox will be performed by the Minnesota Department of Health and/or the Centers for Disease Control and Prevention.

The smallpox risk level should be clearly noted on the laboratory requisition form accompanying any specimen labeled as “vesicle,” “blister,” “rash,” or otherwise suggestive of acute/generalized vesicular or pustular rash illness.

Turn Around Time: Preliminary PCR results within 1 day for vaccinia, VZV and non variola panel

Refer to Arboviral Encephalitis Panel

Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

Our laboratory participates in a project for the Antimicrobial Resistance Laboratory Network (ARLN). Isolate will be forwarded to the Minnesota Public Health Laboratory for serotyping and susceptibility testing.

*A report with serotyping results is available upon request.

Acceptable Specimen: 2 ml serum

This panel starts with chemiluminescent microparticle immunoassay screen (CMIA). If positive cases are identified, follow up testing will include RPR, and TPPA as needed.

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

Turn Around Time: Nonreactive - 1 day, Reactive - Tuesday and Thursday

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Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: Toxoplasma < 1:16
  CMV < 1:8
  Herpes - Negative
  Rubella - Negative

Turn Around Time: 1 day

Acceptable Specimen: 2 ml acute phase serum. Include immunization history on test request form.

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: <1:16

Turn Around Time: 1 day

Acceptable Specimen: 2 ml serum

Stability: Ambient: 3 days; Refrigerated: 7 days; Frozen: >7 days

Normal Value: Nonreactive

Turn Around Time: Tuesday and Thursday

Acceptable Specimen: 1.5 mL serum, 1.0 mL urine, CSF and amniotic fluid

Must submit Zika Virus patient data form with each patient submission.

*Must meet CDC requirements.

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: Zika Negative, Chikungunya Negative, Dengue Negative

Turn Around Time: 7 days

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Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

A report with susceptibility to vancomycin will be issued to the submitting facility.

Acceptable Specimen: 2 ml serum

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: >1:16

Turn Around Time: Tuesday and Thursday

Acceptable Specimen: 2 ml acute phase serum

Stability: Ambient: 1 day; Refrigerated: 7 days; Frozen: >7 days

Normal Value: <1:8

Turn Around Time: 1 day

Acceptable Specimen: Genital, urethral, oral, tissue, and vesicle in viral transport medium. CSF in sterile container.

Stability: Refrigerated: 10 days; Frozen: >10 days

Normal Value: Negative

Turn Around Time: Tuesday and Friday

Acceptable Specimen: 1 ml spinal fluid

Stability: Ambient: 2 days; Refrigerated: 14 days; Frozen: >14 days

Normal Value: Nonreactive

Turn Around Time: Friday

Mandatory Reportable Condition click HERE to report

Mandatory Isolate Submission

Acceptable Specimen: Pure isolate in appropriate tubed medium or Amies (with charcoal) transport medium. Plates not accepted.

A report with the organism identification will be issued to the submitting facility.

Acceptable Specimen: Swabs in viral transport medium

Viral cultures are reserved for those viruses that are not offered by PCR at the NDDOH.

Stability: Refrigerated: 7 days; Frozen: >7 days

Normal Value: Negative

Turn Around Time: 14 to 28 days

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Refer to Arboviral Encephalitis Panel

Acceptable Specimen: 2 ml late acute phase serum

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: Negative

Turn Around Time: 3 days

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Acceptable Specimen: Isolate in appropriate tubed transport medium or cut out a piece of agar with growth and send in a sterile container. Plates are not accepted. For primary isolation and PCR, collect blood, tissue aspirate or biopsied tissue.

Transport: Blood at room temperature. Tissue samples at 2 to 8°C

Turn Around Time: 3 to 5 days

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Acceptable Specimen: 1.5 mL serum

Must submit Zika Virus patient data form with each patient submission.

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: Negative

All positive results will be forwarded to CDC for confirmation

Turn Around Time: 7 days

Acceptable Specimen: 1.5 mL serum, 1.0 mL urine

Must submit Zika Virus patient data form with each patient submission.

Stability: Ambient: 8 hours; Refrigerated: 2 days; Frozen: >2 days

Normal Value: Negative

Turn Around Time: 7 days